Figure 1.

gp120 contains redox labile disulfide bonds which are effectively reduced by TCEP (A) Maltose binding protein-tagged gp120 from HIV-1 CN54 (1 μg - MW 120 kDa) was incubated for 30 min with TCEP (2.5 mM), TRX (1.25 μg) or PDI (1 μg) and alkylated using 5 mM Alexa Fluor 633-conjugated maleimide. Samples were separated by non-reducing SDS-PAGE alongside non-reduced protein and PDI and TRX enzyme only as controls. (B) The integrated intensity of protein bands was quantified using LICOR Odyssey software. Data shown are representative of 3 separate experiments.