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(A) Analysis of the products of lupin galactan degradation by GanB after 30 min and up to 23 h using thin-layer chromatography. As a control (co.), a mixture of galactose (20 μg) and galactan (5 μg) was applied. (B and C) HPLC profiles of the products of lupin galactan degradation by purified GanB after 30 min (B) and 60 min (C). The separation of the galacto-oligosaccharides was performed by HPLC with pulsed amperometric detection as described in Materials and Methods.
