. 2016 Sep 22;198(20):2853–2863. doi: 10.1128/JB.00262-16

TABLE 2.

Plasmids used in this study

Plasmid	Description and/or use	Source or reference
pACYC184	Amplification of cat cassette	New England Biolabs
pSMART-HCAmp	High-copy-no. cloning vector; Amp^r	Lucigen
pACBSR	Gene-gorging plasmid; contains gene encoding I-SceI endonuclease and λ red recombinase genes under control of the P_BAD promoter; temp-sensitive origin of replication; Cm^r	55
pK-HT	Mutagenesis plasmid; contains gene encoding I-SceI endonuclease under control of the P_tet promoter and λ red recombinase genes under control of the P_rha promoter; Amp^r	56
pKDTS	Mutagenesis plasmid; contains gene encoding I-SceI endonuclease under control of the P_tetA promoter and λ red recombinase genes under control of the P_BAD promoter; temp-sensitive origin of replication; Amp^r	9