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Gene clusters encoding monocins. The blue genes represent putative regulatory protein genes, the pink genes encode structural proteins, and the green genes form the lysis cassette encoding holin and lysin. (A) Entire wild-type gene cluster. (B) Holin/lysin deletion construct (sUC-001). (C) Construct under the control of the inducible Phyper-spank promoter (sGL-071). (D) Monocin gene cluster with the tail fiber fusion plus the downstream chaperone (sGL-365).
