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Bactericidal activity of the recombinant monocin M35152-A118. (A) Comparison of wild-type M35152 and M35152-A118 on two different L. monocytogenes strains, showing the switch in bactericidal spectrum upon engineering of the RBP. M35152 killed strain 23074 but not strain 19111, whereas M35152-A118 killed 19111 but not 23074. (B) Comparison of activities of the 4 putative downstream ORF constructs, showing that ORF 21 is required to generate functional M35152-A118.
