FIG 3.

Effect of temperature on the pressure inactivation of the HuNoV GII.1-509 strain. HuNoV stock was processed at a pressure of 200 MPa for 5 min at 4°C, 20°C, or 40°C. Both untreated and treated samples were incubated with PGM-MBs for 30 min at room temperature followed by real-time RT-PCR. Asterisks denote the groups that are significantly different (P < 0.05). The dashed line indicates the detection limit (2 log₁₀ RNA copies/ml).