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Effect of matrix on the pressure inactivation of the HuNoV GII.4-5M strain. HuNoV GII.4-5M stock was inoculated into MEM, PBS, and H2O and treated at 200 MPa for 5 min at 4°C. The surviving viruses were evaluated by a PGM-MB binding assay and real-time RT-PCR. Asterisks indicate that the treated group is significantly different from the untreated control (P < 0.05). The dashed line indicates the detection limit (2 log10 RNA copies/ml).
