TABLE 1.
Additional carbon source (%) | Glycolysis phase (peak 1) |
Metabolic transition phase (peak 2) |
TCA cycle/gluconeogenesis phase (peak 3) |
||||||
---|---|---|---|---|---|---|---|---|---|
Time (h) | Cell growth (OD600) | RLUs | Time (h) | Cell growth (OD600) | RLUs | Time (h) | Cell growth (OD600) | RLUs | |
Nonea | 4.5 | 0.08 | 4.1.E + 06 | 1.5 | 0.08–0.14 | 2.8.E + 05 | 5.5 | 0.14–0.34 | 4.8.E + 05 |
Glucose (0.008) | 6.5 | 0.23 | 4.2.E + 06 | 1.5 | 0.23–0.36 | 4.1.E + 05 | |||
Acetate (0.016) | 4.5 | 0.08 | 5.5.E + 06 | 1.5 | 0.08–0.14 | 2.7.E + 06 | 6 | 0.14–0.46 | 1.4.E + 06 |
Succinate (0.016) | 4.5 | 0.08 | 5.3.E + 06 | 1.5 | 0.08–0.14 | 7.0.E + 05 | 6 | 0.14–0.46 | 8.0.E + 05 |
Citrate (0.016) | 4.5 | 0.08 | 4.0.E + 06 | 1.5 | 0.08–0.14 | 5.1.E + 05 | 6 | 0.14–0.44 | 1.0.E + 06 |
An M9 medium containing glucose (0.002%) and Casamino Acids (0.2%) was used as the standard medium.