TABLE 3.
Mean values and standard deviations of kinetic parameters of dodecylamine germination of untreated and CAP-treated B. subtilis sporesa
| Treatment time | T1 (min) | Tlag (min) | ΔTleakage (min) | Trelease (min) | ΔTrelease (min) | ΔTlysis (min) | % germination (no. of germinated spores) |
|---|---|---|---|---|---|---|---|
| 0 min | 28.9 ± 18.7 | 30.5 ± 19.1 | 1.6 ± 1.9 | 32.9 ± 19.1 | 2.3 ± 0.7 | 1.8 ± 2.1 | 92.3 (199) |
| 30 s | 29.1 ± 22.3 | 31.3 ± 17.7 | 2.2 ± 3.2 | 33.0 ± 23.4 | 2.6 ± 1.0 | 2.1 ± 4.6 | 90.9 (411) |
| 1 min | 29.6 ± 19.2 | 32.0 ± 23.6 | 2.4 ± 1.0 | 34.4 ± 23.3 | 2.4 ± 1.0 | 1.6 ± 1.6 | 87.6 (247) |
| 2 min | 29.2 ± 16.9 | 32.4 ± 19.0 | 2.2 ± 1.5 | 35.5 ± 18.9 | 2.1 ± 1.5 | 1.8 ± 5.1 | 88.5 (243) |
| 3 min | 32.7 ± 25.3 | 35.5 ± 28.6 | 2.8 ± 4.1 | 41.6 ± 16.4 | 2.6 ± 1.4 | 1.0 ± 1.6 | 92.2 (196) |
a
The kinetic germination parameters T1, Tlag, Trelease, ΔTleakage (Tlag − T1), ΔTrelease (Trelease − Tlag), and ΔTlysis (Tlysis − Trelease) were determined for dodecylamine germination of B. subtilis spores with and without CAP treatment as described in Materials and Methods.