TABLE 1.
Light organ colonization assays
| Test straina | Trial no. | Total no. of animals | No. (%) of cocolonized animalsb | CFU competitive index log (CI) ± 95% confidence interval | Total no. of colonized crypts | No. (%) of cocolonized animals with cocolonized cryptsc |
|---|---|---|---|---|---|---|
| WT | 1 | 20 | 20 (100) | −0.09 ± 0.25 | 98 | 15 (75) |
| 2 | 21 | 14 (63) | 0.18 ± 0.32 | 68 | 8 (57) | |
| 3 | 15 | 14 (93) | −0.01 ± 0.29 | 74 | 9 (64) | |
| Δlux mutant | 1 | 14 | 13 (93) | −0.62 ± 0.31 | 60 | 6 (46) |
| 2 | 26 | 23 (88) | −0.38 ± 0.36 | 103 | 14 (61) | |
| 3 | 17 | 15 (88) | −1.05 ± 0.42 | 69 | 8 (53) |
a
For the inoculum of each trial, the indicated YFP-labeled test strain was mixed evenly with the WT control strain labeled with CFP.
b
Data represent animals with light organ homogenates that yielded both CFP- and YFP-labeled CFU were scored as cocolonized. Percentages were calculated on the basis of the number of animals in each trial.
c
Data represent animals with at least one crypt containing both CFP- and YFP-labeled cells. Percentages were calculated on the basis of the number of animals determined to be cocolonized by CFU plating.