FIG 2.

Enzymatic activity of R. anatipestifer rPncA. (A) Schematic of the coupled assay for measurement of the enzyme kinetics of rPncA. GIDH, glutamate dehydrogenase. (B) Assay results. One microgram of purified rPncA was added to reaction buffer to measure the enzyme kinetics. The optical density at 340 nm (OD₃₄₀) decreased similarly with time for the rPncA reaction product and the ammonia standard solution, whereas no change was observed for the reaction buffer without NAM. Distilled water was used as the blank control.