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Immunoblot analysis of the subcellular location of PncA in R. anatipestifer. An antibody against the TonB-dependent receptor of R. anatipestifer was used to control for protein loading and membrane localization. Lane M, molecular size markers; lane 1, whole-cell proteins from R. anatipestifer strain Yb2; lane 2, membrane fraction from R. anatipestifer strain Yb2; lane 3, cytoplasmic fraction from R. anatipestifer strain Yb2.
