TABLE 2.
Oligonucleotides used in this study
| Target | Gene | Oligonucleotide | Sequence (5′–3′)a |
|---|---|---|---|
| Total Clostridium difficile (A+ B+, A− B+, and A− B+ types) | 16S rRNA gene | CD16SrRNA-F | GCAAGTTGAGCGATTTACTTCGGT |
| CD16SrRNA-P | FAM-TGCCTCTCAAATATATTATCCCGTATTAG-TAMRA | ||
| CD16SrRNA-R | GTACTGGCTCACCTTTGATATTYAAGAG | ||
| TcdB-producing Clostridium difficile (A+ B+ and A− B+ types) | tcdB | tcdB-F | TACAAACAGGTGTATTTAGTACAGAAGATGGA |
| tcdB-P | FAM-TTTKCCAGTAAAATCAATTGCTTC-TAMRA | ||
| tcdB-R | CACCTATTTGATTTAGMCCTTTAAAAGC | ||
| TcdA-producing Clostridium difficile (A+ B+ type) | tcdA | tcdA-F | CAGTCGGATTGCAAGTAATTGACAAT |
| tcdA-P | FAM-TTGAGATGATAGCAGTGTCAGGATTG-TAMRA | ||
| tcdA-F | AGTAGTATCTACTACCATTAACAGTCTGC |
a
All oligonucleotides were described previously (16). FAM, 6-carboxyfluorescein; TAMRA, 6-carboxymethyltetramethylrhodamine.