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. 2016 Sep 16;82(19):5795–5805. doi: 10.1128/AEM.01383-16

FIG 2.

FIG 2

Identification of streptothricins and borrelidin from S. rochei Sal35. (a) Chemical structures of streptothricins and borrelidin produced by S. rochei Sal35. (b) PCR amplification of specific DNA fragments in the stt and bor pathways from S. rochei Sal35 genomic DNA. The DNA marker was the 1-kb DNA ladder from Dongsheng Biotech, and the indicative sizes of the bands are 2, 1.5, and 1 kb in panel b. Sequences of PCR primers are listed in Table 2. (c) Extracted ion chromatogram spectra of streptothricins produced by S. rochei Sal35 fermented on SFM, GYM, YBP, and R3 media. ST-F (m/z, 503.4), ST-E (m/z, 631.6), ST-D (m/z, 759.7), and ST-C (m/z, 887.8) were detected and compared to the streptothricin standards; the mass spectra of each compound are shown in Fig. S2 in the supplemental material. (d) HPLC profiles of borrelidin production in S. rochei Sal35 fermented on SFM, GYM, YBP, and R3 media. AU, absorbance units. Note the different scale on the y axis.