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. 2016 Sep 16;82(19):5795–5805. doi: 10.1128/AEM.01383-16

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FIG 3 — LEXAS screening of S. rochei Sal35 BAC genomic library revealed the heterologous expression of the background stt pathway. (a) Selected microferments and bioassay plates from the LEXAS screening procedure. The positive 8E6 BAC exconjugant exhibited strong antibacterial activity against M. smegmatis mc²155. (b) PCR verification of the stt pathway in 2B8 and 8E6. Genomic DNA (gDNA) was set as the control. (c) Comparative metabolic profiling of S. coelicolor M1154/8E6 and S. coelicolor M1154/pHL921 propagated in R3 medium. EIC, extracted ion chromatography. Note the different scale on the y axis.