FIG 1.

Phylogenetic analysis and structure of NanS and NanS-p protein sequences in E. coli O157:H7 strain EDL933. (A) The UPGMA tree of NanS and the NanS-p family was calculated with Mega6 (25) based on the amino acid (aa) sequences. (B) Structural organization of NanS and the NanS-p family. Potential functional domains (DUF, domain of unknown function) were predicted using BLAST. Horizontal boxes with black diagonal stripes depict DUF1737; white horizontal boxes indicate DUF303. Amino acid sequence blocks typical of the SGNH enzyme family of hydrolases as described previously (18) are depicted as vertical lines I, II, III, and IV. Thin horizontal continuous lines illustrate continuous amino acid chains.