FIG 3.
Measurement of temperature and pH optima of NanS-p2-His (A), 933Wp42-His (B), and NanS-p4-His (C). The substrate was 4-methylumbelliferyl acetate. Temperature conditions for measurement of pH optimum and buffer system (dot, acetate; square, potassium phosphate; triangle, Tris-HCl) were 40°C or 20°C for panels A and B or panel C, respectively. The temperature optimum was detected in Tris-HCl buffer (pH 7.0). In the case of temperature optima, 100% relative esteraseMuf-Ac activity data represent 50.9 ± 0.9 nkat/mg (A), 35.3 ± 1.3 nkat/mg (B), and 53.1 ± 0.7 nkat/mg (C). In the case of pH optima, 100% relative esteraseMuf-Ac activity data represent 41.5 ± 0.7 nkat/mg (A), 30.3 ± 0.9 nkat/mg (B), and 52.7 ± 0.4 nkat/mg (C).