FIG 5.
Growth curves of E. coli O157:H7 strain EDL933 and selected EDL933 deletion mutants with different numbers of deleted nanS-p alleles. Data represent the results of cultivation in M9 minimal medium with 0.4% Neu5,9Ac2 (A), Neu5Ac (B), or glucose (C) or without a special added carbohydrate source (D) at 37°C and 180 rpm over 8 h. (A and D) The EDL933 wild-type strain and the EDL933ΔnanS, EDL933ΔnanSΔnanS-p6, and EDL933ΔnanSΔnanS-p1a-p5 mutant strains grew well to approximately the same optical density (A), while the EDL933ΔnanSΔnanS-p1a-p6 and EDL933ΔnanSΔnanS-p1a-p7 mutant strains grew just up to the basal optical density (A and D). (B and C) All mutants grew as well as the wild-type strain on the positive controls with Neu5Ac (B) or glucose (C) as the carbon source. (D) Basal growth was measured in the negative control, where no carbon source had been added.