FIG 5.

Secondary fungemia is initiated by both lytic and nonlytic release of yeast into the bloodstream. (A) Cryptococci within a peripheral endothelial cell after spore inoculation are released into the bloodstream without host cell lysis. The red channel signal representing endothelial cytoplasm rearranges abruptly but does not dissolve, consistent with release without cell lysis. The image was taken 5 days after spore inoculation into Tg(flk:mCherry, mpeg:H2B-egfp) larvae. These are still images from Movie S5 in the supplemental material; the time elapsed from the beginning of the movie (in hours and minutes) is shown in the first panel of each row. White arrowheads indicate the extent of the containing cell. Solid brackets indicate the extent of an intracellular cluster of germinated spores. The dashed bracket indicates the extent of spreading by germinated spores immediately after release. (B) Cryptococci within a complex of endothelial and macrophage cytoplasm are released into the bloodstream with apparent host cell lysis. The green signal representing macrophage cytoplasm dissolves abruptly, consistent with lysis of the host cell. Minutes later, the red channel signal representing endothelial cytoplasm also dissolves. The image was taken 5 days after spore inoculation into Tg(flk:mCherry, mpeg:dendra) larvae. These are still images from Movie S6 in the supplemental material; the time elapsed from the beginning of the movie (in hours and minutes) is shown in the first panel of each row. Magenta arrowheads indicate the edge of the endothelial compartment. Green arrowheads indicate the edge of the enclosing macrophage.