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. 2016 Sep 19;84(10):3083–3091. doi: 10.1128/IAI.00639-16

FIG 1.

FIG 1

The ST receptor GUCY2C is expressed in mouse and human enteroids. (A) Detection of GUCY2C (red) by the monoclonal antibody MS20 in wild-type mouse enteroids costained with antiguanylin antibody (green) and 4′,6-diamidino-2-phenylindole (DAPI; blue). RT-PCR mRNA quantification results (means ± SEM) are shown for GUCY2C and its endogenous peptide ligands guanylin and uroguanylin. Immunoblot analysis results are shown for GUCY2C and actin (loading control) expression in Gucy2c+/+ and Gucy2c−/− mouse enteroids; the SuperSignal molecular mass protein ladder (L; catalog number 84785; Life Technologies) was used to generate the luminescent molecular mass bands. (B) In parallel, detection of GUCY2C (red) by the monoclonal antibody MS20 in healthy human enteroids costained with antiguanylin antibody (green) and DAPI (blue) was performed. RT-PCR mRNA quantification results (means ± SEM) of GUCY2C and its endogenous peptide ligands guanylin and uroguanylin are shown. Immunoblot analysis results are shown for GUCY2C and GAPDH (loading control) expression in healthy human enteroids and HEK293 cells.