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. 2016 Sep 19;84(10):3083–3091. doi: 10.1128/IAI.00639-16

FIG 3.

FIG 3

An ST analog induces secretion in mouse and human enteroids. (A) Representative bright-field microscopy images. Bars, 30 μm. Values below images reflect quantification of swelling (means ± SEM) of Gucy2c+/+ mouse enteroids in response to TJU (control), ST (1 μM), or linaclotide (1 μM) and Gucy2c−/− mouse enteroids in response to TJU (control), linaclotide (1 μM), or 8-Br-cGMP (10 μM). (B) Gucy2c+/+ mouse enteroid swelling occurs in a time- and dose-dependent manner with linaclotide treatment (1 μM and 10 μM, respectively). (C) Normalized swelling dose responses of Gucy2c+/+ mouse enteroids after 2 h of treatment with ST (top) or linaclotide (bottom). (D) Representative bright-field microscopy images (top) and quantification of swelling (means ± SEM) (bottom) in a time-dependent manner after linaclotide treatment (10 μM) of healthy human enteroids. Bars, 30 μm. ****, P < 0.0001.