FIG 4.
ST-mediated secretion can be inhibited by CFTR inhibition, providing proof of concept for testing inhibitors of ETEC. (A) Representative bright-field microscopy images and quantification of results of a normalized swelling assay (means ± SEM) of linaclotide-induced (1 μM) Gucy2C+/+ mouse enteroids pretreated with dimethyl sulfoxide (DMSO) or CFTRinh-172 (50 μM). Bars, 30 μm. (B) In parallel, representative bright-field microscopy images and normalized swelling assay results (means ± SEM) of linaclotide-induced (10 μM) healthy human enteroids pretreated with DMSO or CFTRinh-172 (50 μM). Bars, 30 μm. ***, P < 0.001; ****, P < 0.0001. Data represent individual enteroid measurements derived from 2 to 3 independent samples, which were combined to compose the final data set.