FIG 7.

Production of T-cell-polarizing cytokines by DCs following stimulation with OMVs. Human monocyte-derived DCs were stimulated with 1 μg/ml OMVs for 20 to 24 h. Supernatants were collected from OMV-stimulated DCs, and IL-10 (A), IP-10 (B), IL-23 (C), and IL-12(p70) (D) production was quantified by ELISA (IL-10 and IP-10) or MILLIplex assay [IL-23 and IL-12(p70)]. Data are expressed as the mean ± SEM for four individual donors. Statistical significance was determined using a paired t test between two groups. *, P < 0.05; **, P < 0.01.