FIG 1.

Expansion of bone marrow granulocyte monocyte precursors in mice with an altered microbiota persists after the introduced bacteria are cleared. (A) Male CBA/J mice were colonized with SFB at 5 weeks of age and followed until colonization was cleared. (B) Bone marrow precursors, including granulocyte-monocyte progenitors (CFU-GM), erythroid progenitors (BFU-E), and multipotential granulocyte, erythroid, macrophage, and megakaryocyte progenitors (CFU-GEMM), were measured during acute SFB colonization, 2 weeks after SFB treatment (7 weeks of age), and 2 months after SFB was cleared (8 months of age). (C) Serum levels of SAA were also measured at these time points. (D and E) Bone marrow expression of Jmjd3 (D) and Csf2ra (E) was also determined at these time points. Results are means ± standard errors of the means (SEM) from a representative experiment of two independent experiments with five mice per group. *, P < 0.05.