FIG 5.

Serum amyloid A (SAA) provides protection from E. histolytica infection in an H3K27 demethylase-dependent manner. Mice were injected intraperitoneally with recombinant SAA (days 1, 3, 5) with or without an inhibitor of Jmjd3, GSK-J4, or its inactive control GSK-J5. Mice were then infected with E. histolytica 9 days after the final injection of SAA (day 14) and sacrificed 7 days later (day 21). (A) Bone marrow was collected at day 21, and bone marrow precursors were determined. (B to G) Intestinal expression of il17a (B) Il23p19 (C), and Il22 (D), CD4⁺ T cells (E), neutrophils (F), and infection burden (G) were measured (day 21). Results are means ± SEM from a representative experiment of three independent experiments with 5 (A) or 12 (B to G) mice per group. *, P < 0.05.