FIG 4.
Presence of specific MgtC antibodies in the serum of CF patients infected with M. abscessus. Sera (diluted 1:1,000) from two CF patients infected with M. abscessus and three CF patients negative for infection with M. abscessus were tested using Western blotting against a total lysate of M. smegmatis overexpressing the recombinant MgtCMAB protein tagged with a C-terminal polyhistidine (lanes MgtC) or control M. smegmatis lysate (lanes C). Serum from mice immunized with mgtCMAB DNA against the M. smegmatis lysate overexpressing the recombinant MgtCMAB protein is included as a positive control (right lane). The blots are issued from a single membrane and hybridized with different sera. MW, molecular weight.