FIG 6.
Pathogenic N. meningitidis strains and carrier isolates influence cyclin D1 and cyclin E protein levels. (A) Detroit 562 cells were either left uninfected (control) or infected with N. meningitidis MC58, 8013/clone 12, α711, or α4 (MOI 100) for 24 h. Cell lysates of control and N. meningitidis-infected cells were collected and analyzed for cyclin D1 and cyclin E protein expression by immunoblot analysis. β-Actin was used to normalize protein loading. The figure shows a representative Western blot with lanes from different areas of the same blot. (B) Bar diagram showing the fold increase in the normalized mRNA expression of cyclin D1 and cyclin E in Detroit 562 cells infected with MC58 or α711 in comparison to uninfected control cells. Real-time PCR data were analyzed according to the comparative ΔCT method by first normalizing the sample values (cyclin D1 and cyclin E) to the reference gene values (GAPDH) in infected and uninfected control cells, respectively, and then calculating the relative change in expression (as a fold increase) in infected compared to uninfected cells. Experiments were performed three times, and error bars represent the standard errors of the mean (ns, not significant).