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. Author manuscript; available in PMC: 2016 Sep 27.
Published in final edited form as: J Am Chem Soc. 2016 Sep 7;138(37):12243–12251. doi: 10.1021/jacs.6b06845

Figure 2.

Figure 2

Proteinase K digestion of CP-Ser and variants in the absence and presence of metal ions monitored by analytical HPLC (10–40% MeCN with 0.1% (v/v) trifluoroacetic acid (TFA) over 15 min followed by a gradient of 40–45% MeCN with 0.1% (v/v) TFA over 20 min at 1 mL/min 220 nm absorbance). (A) HPLC chromatograms of 30 μM CP-Ser incubated with 0.45 μM proteinase K in the presence of 1.5 mM Ca(II). (B) HPLC chromatograms of 30 μM CP-Ser and variants incubated with 0.45 μM proteinase K in the presence of 30 μM Mn(II) and 1.5 mM Ca(II). (C) HPLC chromatograms of 30 μM CP-Ser and variants incubated with 0.30 μM proteinase K in the presence of 60 μM Zn(II) and 1.5 mM Ca(II). In panels (B) and (C), the t = 0 h chromatogram is in black, and the t = 2 h chromatogram is in red. The gray regions denote peaks corresponding to the S100A8 and S100A9 subunits. Asterisks indicate the products of proteinase K cleavage.