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. Author manuscript; available in PMC: 2016 Sep 27.
Published in final edited form as: J Am Chem Soc. 2016 Sep 7;138(37):12243–12251. doi: 10.1021/jacs.6b06845

Figure 5.

Figure 5

Zn(II) competition with colorimetric and fluorescent probes. (A) Colorimetric response of 20 μM Zincon to Zn(II) with 2.5 μM CP variant and 250 μM Ca(II) in 75 mM HEPES, 100 mM NaCl, pH 7.0 at 25 ° C. Three independent titrations were performed for each sample, and the mean ± SDM are reported (n = 3). (B) Emission response of 2 μM ZP4 to Zn(II) in the presence of 5 μM CP and 250 μM Ca(II) in 75 mM HEPES, 100 mM NaCl, pH 7.0 at 25 °C. CP-Ser (black circles) and AAA (red squares) outcompete ZP4 for up to 2 equivalents Zn(II). The mean ± SDM are reported (n = 3). (C) Emission response of 2 mM HNBO-DPA with 5 μM CP-Ser, AAA, AHA, H103A, H104A, or H105A and 250 μM Ca(II) upon addition of 0.75 (light gray bars) and 1.50 equivalents of Zn(II) (dark gray bars) in 75 mM HEPES, 100 mM NaCl, pH 7.0 at 25 °C. Mean ± SDM are reported (n = 3). (D) Emission response of 2 μM HNBO-DPA to Zn(II) in the presence of 5 μM CP-Ser or AAA in 75 mM HEPES, 100 mM NaCl, 250 μM Ca(II), pH 7.0 at 25 °C. The mean values are reported (n = 3). The fits are shown as lines.