FIG 7.

Knockdown of SIRT4 alone could phenocopy the effects of MTPα-3K mutant expression in mouse livers. (A and B) Male C57BL/6J mice fed an HF/HS diet for 2 months were injected once a week with adenovirus harboring shNC or shSIRT4. Measurements were performed at 15 days postinfection. The mRNA levels and protein levels of SIRT4 and MTPα in the livers were determined by RT-qPCR (A) and Western blotting (B), respectively. (C) MTPα-3K acetylation levels were compared to MTPα protein levels. (D) Plasma β-hydroxybutyrate levels. (E) Organ weights. (F) Representative images of H&E staining and Oil Red O staining of liver sections (magnification, ×100). (G) Triglyceride and cholesterol levels in livers. (H) Levels of AST and ALT in plasma. (I) Expression levels of inflammatory genes in livers. (J) GTT (left) and ITT (right) results. *, P < 0.05 versus shNC. n = 6 to 10/group. The error bars indicate SD.