FIG 5.

AJUBA LIM proteins do not disrupt MST2-WW45 and MOB1A-LATS2 interactions in cells. (A to C) HEK293T cells were transfected with epitope-tagged plasmids expressing WW45 or MST2 in the absence or in the presence of increasing amounts of LIMD1. The cells were lysed, and either WW45 or MST2 was immunoprecipitated. The bound products were Western blotted with the indicated antibodies. The left columns are input controls (10% of the amount of cell extract immunoprecipitated). (D and E) HEK293T cells were transfected with epitope-tagged plasmids expressing LATS2 or MOB1A in the absence or in the presence of increasing amounts of LIMD1. The cells were lysed, and LATS2 was immunoprecipitated. The bound products were Western blotted with the indicated antibodies. The left columns are input controls (10% of the amount of cell extract immunoprecipitated).