Table I.
Sequences of the primers used in reverse transcription-quantitative polymerase chain reaction.
| Gene (transcript number) | Sequence (5′-3′) | Product size (bp) |
|---|---|---|
| ABCG2 | F: CACGTGATTCTTCCACAAGCC | 74 |
| (NM_004827) | R: CATGTACTGGCGAAGAATATTTGGT | |
| Bmi1 | F: AAATGCTGGAGAACTGGAAAG | 124 |
| (NM_005180) | R: CTGTGGATGAGGAGACTGC | |
| E-cadherin | F: ATTCTGATTCTGCTGCTCTTG | 400 |
| (NM_004360) | R: AGTAGTCATAGTCCTGGTCTT | |
| GAPDH | F: AATTGAGCCCGCAGCCTCCC | 153 |
| (NM_002046) | R: CCAGGCGCCCAATACGACCA | |
| Nanog | F: ATTCAGGACAGCCCTGATTCTTC | 76 |
| (NM_024865) | R: TTTTTGCGACACTCTTCTCTGC | |
| N-cadherin | F: CTCCTATGAGTGGAACAGGAACG | 121 |
| (NM_001792) | R: TTGGATCAATGTCATAATCAAGTGCTGTA | |
| Oct4 | F: GTGGAGAGCAACTCCGATG | 86 |
| (NM_002701) | R: TGCTCCAGCTTCTCCTTCTC | |
| Snail | F: GAGGCGGTGGCAGACTAG | 159 |
| (NM_005985) | R: GACACATCGGTCAGACCAG | |
| Sox2 | F: CGAGTGGAAACTTTTGTCGGA | 74 |
| (NM_003106) | R: TGTGCAGCGCTCGCAG | |
| Twist | F: CGGGAGTCCGCAGTCTTA | 130 |
| (NM_000474) | R: TGAATCTTGCTCAGCTTGTC |
F, forward; R, reverse; ABCG2, ATP-binding cassette sub-family G member 2; Bmi1, B lymphoma Mo-MLV insertion region 1 homolog; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; Oct4, octamer-binding transcription factor 4; Sox2, sex determining region Y-box 2.