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. 2016 Sep 22;34(41):4955–4963. doi: 10.1016/j.vaccine.2016.08.048

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© 2016 The Author(s)

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

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Fig. 5 — Priming with Ad26 vector results in improved recall responses after MVA or Ad35 boosting compared to priming with Ad5 vector. (A) Experimental outline. (B) Representative FACS plots showing the percentage of Gag-specific CD8 T cells before and after MVA boost in blood. (C) Summary showing the number of Gag-specific CD8 T cells before and after MVA boost in blood. (D) Summary showing the fold-increase of Gag-specific CD8 T cells in blood after MVA boost. (F) MFI of IFN-γ expression by Gag-specific CD8 T cells from liver. (F) Representative FACS plots showing functionality of Gag-specific CD8 T cells in liver as measured by tetramer and cytokine expression. (G) Summary showing the number of Gag-specific CD8 T cells before and after Ad35 boost in blood. Unprimed mice vaccinated with MVA or Ad35 were used as controls (Unvax). Data are representative of two independent experiments, with n = 4 mice per group per experiment. ^*p < 0.05; ^**p < 0.01.