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. 2016 May 18;7(5):558–577. doi: 10.1080/21505594.2016.1163458

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Figure 3. — Mapping glycan epitopes in cek1 mutants. (A) Different cell wall fractions from stationary (st) and exponential (x) phase cultures of CAF2 and cek1 strains were separated by SDS-PAGE before blotting with 5B2 (β‑M) and EB-CA1 (α‑M) antibodies. CWMP extracts were released by heat treatment (Fraction 1), NaOH (Fraction 3) or zymolyase treatment (Fraction 4). (B) Zymolyase released CWMP extracts (fraction 4) from CAF2 and cek1 cells grown at exponential phase in RPMI media were separated by SDS-PAGE and analyzed by western blotting with the MAb 16B.1 for the detection of Hwp1. The predicted glycosylated form of Hwp1 is highlighted with an arrow. (C) Zymolyase (Fraction 4) extracts from the indicated strains grown at exponential phase in YEPD or RPMI media were analyzed with the mAb 2g8 for the detection of β‑1,3‑glucan.