Figure 5.

Effect of cucurbitacin B on activation of JAK2/STAT3 signaling pathway. MKN-45 cells were treated with 0.1, 1 or 10 µM cucurbitacin B for 24 h. Phosphorylation of JAK2 and STAT3 was analyzed by western blot analysis. (A) Representative western blot analysis and (B) quantitative results. Experiments were repeated at least three times. Data are expressed as the mean ± standard deviation. *P<0.05, ^#P<0.01 vs. control group. p-, phosphorylated; STAT3, signal transducer and activator of transcription 3; JAK2, Janus kinase 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Ctrl, control.