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. 2016 Sep 6;12(4):2651–2656. doi: 10.3892/etm.2016.3672

Figure 3.

Figure 3.

Wnt5a is the direct target of miR-129-5p in SMCs. (A) Effects of miR-125-5p on the expression of endogenous Wnt5a mRNA, as tested by reverse transcription-quantitative polymerase chain reaction. (B) Western blot analysis was used for monitoring Wnt5a expression in SMCs 48 h after transfection with miR-125-5p. (C) Binding between miR-125-5p and the miR-125-5p-binding site in the 3′UTR of Wnt5a. (D) Luciferase reporter assay with cotransfection of wild-type or mutant 3′UTR and miR-125-5p or miR-control in SMCs. Firefly luciferase activity of each sample was normalized against Renilla luciferase activity. Data from three separate experiments are presented as mean ± standard deviation. **P<0.01 vs. miR-control. miR, microRNA; SMC, smooth muscle cell; 3′UTR, 3′ untranslated region.