Figure 3.

Effect of Pre-miR-143 transfection on the HeLa cell cycle. (A) Fixed cells were treated with RNase and stained with propidium iodide. The representative figures of the blank (left panel), negative control (middle panel) and pre-miR-143 incubated with HeLa cells (right panel) for cell cycle analysis via flow cytometry are presented. The proportion (%) of G0/G1, S and G2/M phases of the cell cycle are presented for (B) 20 nM or (C) 40 nM concentrations of blank, negative control or Pre-miR-143 incubated with HeLa cells. For each analysis, a minimum of 10,000 cells was counted. miR, microRNA; pre-miR-143, precursor miR-143; CV, coefficient of variance.