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. 2016 Jul 27;12(4):2567–2573. doi: 10.3892/ol.2016.4914

Figure 2.

Figure 2.

Screening of miR-486-5p in HCC tissues and Huh-7 cells, and the impact of ectopic expression of miR-486-5p on IGF-1R and its downstream mediators in Huh-7 cells. (A) Screening of miR-486-5p in liver tissues showed significant downregulation in the liver tissues obtained from the HCC patients (P=0.0022) and in the Huh-7 cells (P=0.0121) when compared with normal liver tissues. (B) Forcing the expression of miR-486-5p in Huh-7 cells resulted in the repression of IGF-1R mRNA expression compared with that of the mock cells (P=0.0003). Similarly, STAT3, mTOR and c-Myc mRNA levels were also found to be significantly downregulated compared with those of the mock cells (P=0.0472, P=0.0086 and P=0.0159, respectively). *P<0.05, **P<0.01 and ***P<0.001. HCC, hepatocellular carcinoma; IGF-1, insulin-like growth factor 1; IGF-1R, IGF-1 receptor; mTOR, mammalian target of rapamycin; STAT, signal transducer and activator of transcription; miR, microRNA; RQ, relative quantification.