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Confocal images of representative E. coli spheroplasts incubated with FITC-labeled peptides (BF2, P11A BF2, HipC, or magainin 2) and di-8-ANEPPS. Images from the middle of a z-stack of each spheroplast were chosen, and the merged fluorescence of FITC (green) and di-8-ANEPPS (red) is shown.
