TABLE 1.
Peptide | Sequence | No. of spheroplasts | % translocating | % membrane localized |
---|---|---|---|---|
BF2 | TRSSRAGLQWPVGRVHRLLRK | 67 | 63 | 37 |
P11A BF2 | TRSSRAGLQWAVGRVHRLLRK | 101 | 26 | 74 |
Magainin 2 | GIGKWLHSAKKFGKAFVGEIMNS | 60 | 18 | 82 |
HipC | GNYAHRVGAGAPVWL | 67 | 97 | 3 |
Data for each peptide were collected from at least two independently prepared batches of spheroplasts, characterized over a total of at least five separate imaging sessions for each peptide; data for each spheroplast batch are given in Tables S1 to S4 in the supplemental material.