FIG 3.

Analysis of extracellular exposure of phosphatidylserine by annexin V/propidium iodide labeling. The parasites were treated with MB17, MB19, and MB38 at concentrations corresponding to the IC₅₀s (0.5 ± 0.13, 3.7 ± 0.29, and 8.3 ± 1.49 μM, respectively) and IC₈₀s (1.5 ± 0.5, 8.0 ± 0.94, and 16 ± 3.2 μM, respectively) for 5 days. After this period, the parasites were labeled with annexin V and propidium iodide and analyzed by flow cytometry as follows: untreated epimastigotes (a), parasites treated with MB17 at concentrations corresponding to the IC₅₀ (b) and IC₈₀ (e), parasites treated with MB19 at concentrations corresponding to the IC₅₀ (c) and IC₈₀, (f), and parasites treated with MB38 at concentrations corresponding to the IC₅₀ (d) and IC₈₀ (g). Quantitative analysis of the parasites treated with MB17, MB19, and MB38, as indicated, are also shown. One-way ANOVA followed by a Tukey posttest was used for statistical analysis to compare the treatments to values in the respective control. ***, P < 0.001; **, P < 0.01 (Tukey posttest).