FIG 4.

Fluorescence microscopy analysis of the intracellular distribution of MB17. Parasites were treated with 4 μM MB17, fixed with 4% paraformaldehyde, incubated with Hoechst, and analyzed by light microscopy (a, f, k, and p) and by fluorescence for Hoechst staining (b, g, l, and q) and MB17 staining (c, h, m, and r). Merged images for MB17 and Hoechst (d, i, n, and s) and for light microscopy, MB17, and Hoechst (e, j, o, and t) were obtained. Magnifications: ×1,000 (a to o) and ×600 (p to t).