FIG 5.
Effect of MB17 on epimastigote cell cycle using fluorescence-activated cell sorting. Control cells and cells treated with MB17 (IC50) or MB17 (IC80) for 5 days were treated with RNase A and stained with propidium iodide, and their DNA content was analyzed by fluorescence-activated cell sorting. Histograms (a) and quantifications of the numbers of cells in each stage of the cell cycle (b) are shown. Representative histograms of three independent experiments are shown, and results are compared between control and MB17 (IC50)-treated G1-phase cells and between control and MB17 (IC80)-treated G1-phase cells. **, P < 0.01; *, P < 0.05 (Student's t test).