FIG 2.

Effects of SQ109 on L. mexicana and J774 macrophages. (A) Promastigote growth as a function of time and SQ109 concentration. (B) Effects of SQ109 on the viability of L. mexicana promastigotes. The IC₅₀ (after 72 h of treatment) is 0.53 ± 0.06 μM. Miltefosine is shown as a positive control. The asterisks represent statistically significant differences, determined using the Student t test, P ≤ 0.05 (*) and P ≤ 0.01 (**). (C) Effects of SQ109 on J774 macrophage viability; IC₅₀ (after 72 h of treatment) is 5.8 ± 0.1 μM. (D) Effects of SQ109 on macrophages infected with L. mexicana amastigotes. SQ109 potently inhibits amastigote proliferation, yielding an IC₅₀ (after 48 h of treatment) of 11 ± 0.9 nM. The protocols used in panels A and D are the same as those described in reference 5 and for panels B and C in reference 16 (the error bars represent standard deviation for least three independent experiments).