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Effects of SQ109 and FCCP on the mitochondrial electrochemical potential and SQ109 on Ca2+ flux. (A) SQ109 at 5 μM, followed by 2 μM FCCP using rhodamine 123 as a probe of the electrochemical potential. (B) Same as panel A but FCCP added first and then SQ109. (C) Ca2+i as determined using the radiometric fluorescent Ca2+ indicator fura 2 in the presence of 2 mM external Ca2+. (D) Same as panel C but no external Ca2+ (8 mM EGTA). Methods are as described in reference 5.
