FIG 3.

Impact of MspA-mediated outer membrane permeability on the activity of 8HQs against M. smegmatis SMR5. Dose-response curves of the M. smegmatis wild type (wt), its triple porin mutant ML16, and the MspA-expressing ML16 strain (ML16 mspA) against 8-hydroxyquinoline (A), CQ (B), and NQ (C) in the presence or absence of CuSO₄. Error bars represent standard deviations from at least three sample replicates. Individual data points represent the means from three replicates from the same assay plate. Error bars indicate standard deviations. Background values were subtracted from all values, and all values were normalized to those for the untreated controls.