FIG 1.

Backbone of pICBEC72Hmcr IncX4 plasmid (GenBank accession no. CP015977) carrying the mcr-1 gene in a human E. coli strain isolated in Brazil. The pICBEC72H plasmid was compared with plasmid pmcr1_IncX4 (GenBank accession no. KU761327.1) from a MCR-1-positive ST25 Klebsiella pneumoniae strain isolated from a human clinical sample in China (12), pESTMCR (GenBank accession no. KU743383.1) from a MCR-1-positive E. coli strain isolated from pig slurry in Estonia, and pAF48 (GenBank accession no. KX032520.1) from a MCR-1-positive ST624 E. coli strain isolated from a human clinical sample in South Africa (13). Comparative analysis was performed using Easyfig version 2.2.2 for genome comparisons. White arrows, genes that are part of transfer modules.