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. 2016 Sep 23;60(10):5806–5816. doi: 10.1128/AAC.02877-15

FIG 2.

FIG 2

Raman spectroscopy to characterize the capsule status of A. baumannii strains. A. baumannii strains were grown in LB medium in the absence or presence of phage, pelleted by centrifugation, and analyzed by Raman spectroscopy. The Raman spectra revealed inherent spectral markers for the opaque, capsule-positive strain AB5075 (and AB5075::lux), specifically the Raman spectral bands at 979 cm−1 (A) and 1,422 cm−1 and 1,379 cm−1 (B). These spectral bands were absent in the capsule-negative strains AB5075P, AB5075 epsA::Tn5, AB5075 ptk::Tn5, and streak-purified AB-Army1-treated AB5075 (and AB5075::lux). a.u., arbitrary units.