TABLE 5.

Biofilm cell membrane depolarization and permeabilization activities of RNase 3, RN3(5-17P22-36), GL-13, LL-37, WY-12, and CA-M peptides on established P. aeruginosa biofilms^a

Peptide	Depolarization		Permeabilization
	ED50 (μM) ± SEM	Mean % ± SEM	ED50 (μM) ± SEM	Mean % ± SEM
RNase 3	9.45 ± 0.97	46.11 ± 3.21	8.59 ± 1.70	39.4 ± 2.88
RN3(5-17P22-36)	3.69 ± 0.37	73.51 ± 1.94*	6.60 ± 2.01	64.26 ± 5.44*
GL-13	ND	ND	ND	ND
LL-37	8.83 ± 0.54	31.85 ± 1.15	12.35 ± 3.13	24.21 ± 3.71
WY-12	18.11 ± 0.89	32.58 ± 1.87	18.79 ± 8.23	18.91 ± 2.23
CA-M	15.79 ± 0.77	39.09 ± 2.46	10.64 ± 0.11	23.01 ± 1.13

^aData shown are the ED₅₀s and the percentages of the maximums at the final incubation time for membrane depolarization and permeabilization, calculated as described in Materials and Methods. Results with 10% Triton X-100 were considered the maximum reference values. ND, not detected at the highest assayed concentration (50 μM). Values are means ± standard errors of the means. *, P < 0.05; significant differences were determined by ANOVA with Scheffé and Bonferroni post hoc tests.