FIG 4.

Efflux map of 38 vaginal C. albicans clinical isolates. The controls used were strains 2-76, 12-99, SC5314, and DSY1050 (black circles). The data were normalized to those for strain 2-76. Values were calculated as described in the inset in Fig. 3. Green symbols, FLC-susceptible strains; red symbols, FLC-resistant strains; yellow triangles, FLC resistant-strains overexpressing ERG11; red squares, isolates S14, S36, and S37, which are unusual in showing R6G efflux but not Ala-Nap efflux; green squares, isolates S24 and S25, which are unusual as they are susceptible isolates showing significant efflux by both the Ala-Nap and R6G assays, most likely due to CDR4 overexpression. The results in the region between the vertical dotted lines are not significantly different from those for the control for the Ala-Nap assay, and the results in the region between the horizontal dotted lines are not significantly different from those for the control for the R6G assay. Significance was calculated using one-way analysis of variance with Dunnett's multiple-comparison tests. Error bars and slopes were calculated by using the LINEST function in Microsoft Excel software on curves generated from biological triplicates from both the Ala-Nap and R6G assays.